Exploration of the Antibacterial and Enzymatic Potential of Fungal Associated with Giant Clams (Hippopus hippopus Linnaeus, 1978) in Ambon Waters
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Enzymatic ,
Hippopus hippopus ,
Fungi ,
Secondary metabolites
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Abstract
Marine fungi associated with giant clams (Hippopus hippopus) are a source of secondary metabolites with great potential in the development of antibacterial agents and enzyme producers. Exploration of fungi from marine organisms is important considering the increasing need for new bioactive compounds. This study aims to: (1) determine the potential antibacterial activity against test bacteria, (2) determine the potential proteolytic, amylolytic, and lipolytic enzyme activities. The research methods include isolation of fungi from clam tissue, antibacterial tests using the agar plug method against test bacteria Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa, and Cutibacterium acnes and enzymatic activity tests.Three isolates of giant clam-associated fungi (Hippopus hippopus) were isolated, namely SK1 10.4, SK1 10.6, and SK1 10.6.2. Antibacterial tests of the three isolates showed inhibitory activity against the growth of test bacteria. Isolate SK1 10.6 was the best candidate because it had stable antibacterial activity and a large inhibition zone. In addition, this isolate also showed protease enzymatic activity.
Key word : Antibacterial; Enzymatic; Hippopus hippopus; Fungi; Molecular
Jamur laut yang berasosiasi dengan kima (Hippopus hippopus) merupakan salah satu sumber metabolit sekunder yang berpotensi besar dalam pengembangan agen antibakteri dan penghasil enzim. Eksplorasi jamur dari organisme laut menjadi penting mengingat meningkatnya kebutuhan terhadap senyawa bioaktif baru. Penelitian ini bertujuan untuk: (1) mengetahui potensi aktivitas antibakteri terhadap bakteri uji, (2) mengetahui potensi aktivitas enzim proteolitk, amilolitik, dan lipolitik. Metode penelitian meliputi isolasi jamur dari jaringan kima, uji antibakteri menggunakan metode agar plug terhadap bakteri uji Staphylococcus aureus, Escherichia coli, Staphylococcus epidermidis, Pseudomonas aeruginosa, dan Cutibacterium acnes, serta uji aktivitas enzimatik. Jamur asosiasi kima (Hippopus hippopus) yang diisolasi sebanyak 3 isolat, yaitu SK1 10.4, SK1 10.6, dan SK1 10.6.2. Uji antibakteri ketiga isolat tersebut menunjukkan adanya aktivitas penghambatan terhadap pertumbuhan bakteri uji. Isolat SK1 10.6 sebagai kandidat terbaik karena memiliki aktivitas antibakteri yang stabil dan zona hambat yang besar. Selain itu, isolat ini juga menunjukkan aktivitas enzimatik protease.
Kata kunci : Antibakteri; Enzimatis; Hippopus hippopus; Jamur; Molekuler
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References
Abdulkareem, A. A., Taweel, F. B. A., Sharqi, A. J. B. A., Gul, S. S., Sha, A. Chapple, I. L. C., (2023). Current concepts in the pathogenesis of periodontitis: from symbiosis todysbiosis. Journal of Oral Microbiology, 15(1): 1-19. https://doi.org/10.1080/20002297.2023.2197779
Abe, C. A. L., Faria, C. B., Castro, F. F. D., Souza, S. R. D., Santos, F. C., Silva, C. N. D., Tessmann, D. J. Tessmann, P. B. (2015). Fungi isolated from maize (Zea mays L.) grains and production of associated enzyme activities. International Journal of Molecular Sciences, 16: 15328-15346. https://doi.org/10.3390/ijms160715328
Ayuningtyas, E. P., Sibero, M. T., Hutapea, N. B., Frederick, E. H., Murwani, R., Zilda, D. S., Wijayanti, D. P., Sabdono, A., Pringgenies, D. Radjasa, O. K. (2021). screening of extracellular enzyme from phaeophyceae associated fungi. IOP Conf. Series: Earth and Environmental Science, 750(1): 1-10. 10.1088/1755-1315/750/1/012005
Bahry, M. S., Radjasa, O. K. Trianto, A. (2021). Potential of Marine Sponge-Derived Fungi in the Aquaculture System. Biodiversitas, 22(7):2883-2892. https://doi.org/10.13057/biodiv/d220740
Bakhtra, D., Yanwirasti, Y., Wahyuni, F. S., Aminah, I., Handayani, D. (2022). Antimicrobial and Cytotoxic Activities Screening of Marine Invertebrate-Derived Fungi Extract from West Sumatera, Indonesia. Open Access Macedonian Journal of Medical Sciences, 10: 1427–1432. https://doi.org/10.3889/oamjms.2022.10374
Camenzind, T., Trigueros, C. A. A., Hempel, S., Lehmann, A., Bielcik, M., Linares, D. R. A., Bergmann, J., Cruz, J. D., Gawronski, J., Golubeva, P., Haslwimmer, H., Lartey, L., Leifheit, E., Maab, S., Marhan, S., Pinek, L., Powell, J. R., Roy, J., Veresoglou, S. D., Wang, D., Wulf, A., Zheng, W., Rillig, M. C. (2024). Towards establishing a fungal economics spectrum in soil saprobic fungi. Nature Communications, 15(3321): 1-13. https://doi.org/10.1038/s41467-024-47705-7
Gonçalves, M. F. M., Esteves, A. C., Alves, A. (2022). Marine fungi: opportunities and challenges. Encyclopedia, 2(1): 559–577. https://doi.org/10.3390/encyclopedia2010037
Goncalves, M. F. M., Paco, A., Escada, L. F., Albuquerque, M. S. F., Pinto, C. A., Saraiva, J. A., Duarte, A. S., Santos, T. A. P. R., Esteves, A. C., Alves, A. (2021). Unveiling biological activities of marine fungi: the effect of sea salt. Applied Sciences, 11(13): 1-18. https://doi.org/10.3390/app11136008
Correa, T. A., Santos, F. S., Camargo, M. G., Quinelato, S., Bittencourt, V. R. E. P. Golo, P. S. (2022). Comparison of methods for isolating entomopathogenic fungi from soil samples. journal of visualized experiments, 179: 1-12. https://dx.doi.org/10.3791/63353
Hashem, A. H., Attia, M. S., Kandil, E. K., Fawzi, M. M., Abdelrahman, A. S., Khader, M. S., Khodaira, M. A., Emam, A. E., Goma, M. A. Abdelaziz, A. M. (2023). Bioactive compounds and biomedical applications of endophytic fungi: a recent review. Microbial Cell Factories, 22: 1-23. https://doi.org/10.1186/s12934-023-02118-x
Hawksworth, D. L. Lucking, R. (2017). Fungal Diversity Revisited: 2.2 to 3.8 Million Species. Eukaryotes: Fungi and Parasitology, 5(4): 1-17. https://doi.org/10.1128/microbiolspec.funk-0052-2016
Hengkengbala, S. I., Lintang, R. A. J., Sumilat, D. A., Mangindaan, R. E. P., Ginting, E. L. Tumembouw, S. (2021). Karakteristik morfologi dan aktivitas enzim protease bakteri simbion nudibranch. Jurnal Pesisir dan Laut Tropis, 9(3): 83-94. https://doi.org/10.35800/jplt.9.3.2021.36672
Hossain, T. J. (2024). Methods for Screening and evaluation of antimicrobial activity: a review of protocols, advantages, and limitations. European Journal of Microbiology and Immunology, 14(2): 97-115. https://doi.org/10.1556/1886.2024.00035
Ilesanmi, O. I, A. E Adekunle, J. A Omolaiye, E. M. Olorode, A. L Ogunkanmi. (2020). Isolation, optimization and molecular characterization of lipase producing bacteria from contaminated soil. Scientific African, 8: 1-10. https://doi.org/10.1016/j.sciaf.2020.e00279
Imran, M., Nazar, M., Saif, M., Khan, M. A., Sanaullah, S., Vardan, M., Javed, O. (2016). Role of enzymes in animal nutrition: a Review. PSM Veterinary Research, 1(2): 38-45. https://psmjournals.org/index.php/vetres/article/view/84
Karwehl, S. Stadler, M. (2016). Exploitation of fungal biodiversity for discovery of novel antibiotics. Current Topics in Microbiology and Immunology, 398: 1-36. https://doi.org/10.1007/82_2016_496
Kour, D., Rana, K. L., Yadav, N., Yadav, A. N., Singh, J., Rastegari, A. A. Saxena, A. K. (2019). Agriculturally and industrially important fungi: current developments and potential biotechnological applications. BMC Marine Science, 2: 1-64. https://doi.org/10.1007/978-3-030-14846-1_1
Krain, A., Siupka, P. (2021). Fungal Guttation, a Source of bioactive compounds, and its ecological role—A Review. Biomolecules, 11(9): 1-17. https://doi.org/10.3390/biom11091270
Linz, M. S., Mattappallil, A., Finkel, D. Parker, D. (2023). Clinical Impact of Staphylococcus aureus Skin and Soft Tissue Infections. Antibiotics, 12(3): 1-27. https://doi.org/10.3390/antibiotics12030557
Mies, M. (2019). Evolution, diversity, distribution and the endangered future of the giant clam–symbiodiniaceae association. Coral Reefs, 38: 1067-1084. https://doi.org/10.1007/s00338-019-01857-x
Neo, M. L., Eckman, W., Vicentuan, K., Teo, S. L. M. Todd, P. A. (2015). The ecological significance of giant clams in coral reef ecosystems. Biological Conservation, 181: 111-123. https://doi.org/10.1016/j.biocon.2014.11.004
Santos, M. M., Soares, H. K. S. S., Sousa, A. M., Bezerra, N. P. C., Cantanhede, S. P. D. Serra, I. M. R. S. (2023). Mapping of the world scientific production on bacterial and fungal microbiota in mollusks. Latin American Journal of Aquatic Research, 51(2): 268-281. http://dx.doi.org/10.3856/vol51-issue2-fulltext-2908
Sattar, H., Bibi, Z., Kamran, A., Aman, A. Qader, S. A. U. (2019). Degradation of complex casein polymer: production and optimization of a novel serine metalloprotease from Aspergillus niger KIBGE-IB36.” Biocatalysis and Agricultural Biotechnology, 21: 1-7. https://doi.org/10.1016/j.bcab.2019.101256
Severn, M. M., Horswill, A. R. (2023). Staphylococcus epidermidis and its dual lifestyle in skin health and infection. In Nature Reviews Microbiology, 21(2): 97–111. https://doi.org/10.1038/s41579-022-00780-3
Surya, S., Pringgenies, D., Sedjati, S., (2025). Antibacterial and enzymatic activities of symbiotic bacteria from gastropods and bivalves in marine skincare Applications. International Journal of Design and Nature and Ecodynamics, 20(1): 83–90. https://doi.org/10.18280/ijdne.200109
Taufiq, M. M. J., Darah, I., 2019. Antibacterial activity of an endophytic fungus Lasiodiplodia pseudotheobromae IBRL OS-64 residing in leaves of a medicinal herb, Ocimum sanctum linn. Journal of Applied Biology and Biotechnology, 7(2): 35–41. https://doi.org/10.7324/JABB.2019.70207
Triandiza, T., Zamani, N. P., Madduppa, H., Hernawan, U. E. (2019). Distribution and abundance of the giant clams (Cardiidae: Bivalvia) on Kei Islands, Maluku, Indonesia. Biodiversitas, 20(3): 884-892. https://doi.org/10.13057/biodiv/d200337
Urganci, N. N., Yılmaz, N., Koçer Alaşalvar, G., Yıldırım, Z. (2022). Pseudomonas aeruginosa and Its Pathogenicity. Turkish Journal of Agriculture - Food Science and Technology, 10(4): 726–738. https://doi.org/10.24925/turjaf.v10i4.726-738.4986